对虾一种无包涵体杆状病毒病原的PCR检测   总被引：4，自引：0，他引：4  

石拓  孔杰  刘萍  包振民  韩玲玲  徐怀恕 《海洋学报》2000,22(4):96-100

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.  相似文献   

南麂列岛海洋自然保护区潮间带小型生物初步研究   总被引：3，自引：3，他引：3  

林岿璇  张志南  韩洁 《中国海洋大学学报(自然科学版)》2003,33(2):219-225

20 0 1年 10月对浙江省南麂列岛国家海洋自然保护区潮间带进行小型生物采样调查。大沙岙沙滩小型生物丰度较低 ,中潮带为 2 91.9ind· 10 cm-2 ,低潮带为 5 16.8ind· 10 cm-2 。线虫是最优势类群 ,线虫 /桡足类比分别为 3.2 7和 1.0 6,表明沙滩环境质量良好 ,未受到有机质污染。岩相潮间带有 4种大型底栖海藻。附植小型生物平均丰度为 36.1ind· g-1dw algae,桡足类为最优势类群。附植大型底栖动物丰度 4 4 .6ind· g-1dwt algae,大于小型生物丰度。  相似文献   

Sea surface height anomaly and geostrophic circulation variations in the South China Sea from TOPEX/POSEIDON altimetry     

Liu Kexiu  Ma Jirui  Xu Jianping  Han Guijun  Fan Zhenhua 《海洋学报(英文版)》2002,21(3):345-354

The sea surface height anomaly (SSHA) and geostrophic circulation in the South ChinaSea (SCS) are studied using TOPEX/POSE1DON (T/P) altimetry data. The SSHA, which is obtained after tidal correction based on the tidal results from T/P data, is predominated by seasonal alternating monsoons. The results reveal that the SSHA in the central part of the SCS is positive in spring and summer, but negative in autumn and winter. It is also found that the SSHA in the SCS can be approached with the sum of tidal constituents SA and SSA. The geostrophic circulations in the SCS are calculated according to sea surface dynamic topography, which is the sum of SSHA and mean sea surface height. It is suggested that the circulation in the upper layer of the SCS is generally cyclonic and notably western intensified during autumn and winter, while the western intensification is weak during spring and summer. It is also indicated that the Kuroshio intrudes into the northeastern SCS throuth the Luzon Strait in winter. But ther  相似文献   

东海西北部水团分析及与渔场的关系     

刘树勋  韩士鑫  魏永康 《中国海洋大学学报(自然科学版)》1989,(Z1)

作者用聚类分析法划分浅海水团,目的在于克服大洋水团分析法进行分析所遇到的一些困难,它在根据不同海区特点确定临界值与检验值时有较大的灵活性。本文讨论了浅海变性水团与中心渔场的关系。结果表明,东海底层冷水、东海表层水和大陆沿岸水对渔业生产有重要影响。  相似文献   

鲁苏榴辉岩的地球化学     

韩宗珠 《中国海洋大学学报(自然科学版)》1994,(1)

以微量元素、稀土元素、Ｓｒ和Ｎｄ同位素变异特征为依据，确定鲁苏榴辉岩为多成因、多来源和多阶段，指出主要是在印支期扬子陆块与华北陆块碰撞造山作用过程中，挤入的上地幔碎片以及不同原岩类型的壳内高压变质岩碎块。燕山晚期的区域构造热事件使得某些榴辉岩的同位素体系再平衡。  相似文献   

Geochemistry and environment evolution of Core E in the Laizhou Bay since last stage of Middle Pleistocene     

Han Deliang  Yu Hongjun  Li Guanbao 《海洋学报(英文版)》2001,20(3):391-399

IwrincrIONOn the evolution of palcoenvironment, salt-water encroachmnt and the distributionof subsurface brine in the LaizhOu Bay area, many researches had been carried out, and muchknowledge abeut the gcolOgical features in this area since Late QUaternary was aCquired' ) (H8nand Wu, l992; Han and Meng, l994; Wen et al. l989). However there was IittIe studyon the sediment geOChemical characteristics since Quaternary. In this Paper, the element strati-graphic characteristics and evolut…  相似文献   

Complexation of mercury by dissolved organic matter in surface waters of Galveston Bay, Texas   总被引：3，自引：0，他引：3  

Seunghee Han  Gary A. Gill  Ronald D. Lehman  Key-Young Choe 《Marine Chemistry》2006,98(2-4):156-166

The chemical speciation of dissolved mercury in surface waters of Galveston Bay was determined using the concentrations of mercury-complexing ligands and conditional stability constants of mercury-ligand complexes. Two classes of natural ligands associated with dissolved organic matter were determined by a competitive ligand exchange-solvent solvent extraction (CLE-SSE) method: a strong class (L_s), ranging from 19 to 93 pM with an average conditional stability constant (K_HgLs) of 10²⁸, and a weak class (L_w) ranging from 1.4 to 9.8 nM with an average K_HgLs of 10²³. The range of conditional stability constants between mercury and natural ligands suggested that sulfides and thiolates are important binding sites for dissolved mercury in estuarine waters. A positive correlation between the estuarine distribution of dissolved glutathione and that of mercury-complexing ligands supported this suggestion. Thermodynamic equilibrium modeling using stability constants for HgL, HgCl_x, Hg(OH)_x, and HgCl(OH) and concentrations of each ligand demonstrated that almost all of the dissolved mercury (> 99%) in Galveston Bay was complexed by natural ligands associated with dissolved organic matter. The importance of low concentrations of high-affinity ligands that may originate in the biological system (i.e., glutathione and phytochelatin) suggests that the greater portion of bulk dissolved organic matter may not be important for mercury complexation in estuarine surface waters.  相似文献   

利用X射线衍射测定单斜辉石中硬玉组分含量     

王文正  韩宗珠  安英杰 《海洋湖沼通报》1993,(4):45-48

硬玉是单斜辉石中十分重要的端元组分,是单斜辉石分类的主要基础。利用x射线衍射分析确定的硬玉组分含量计算式为Jd(％)=[A-2.235]×25.741,其中Jd为硬玉组分百分含量,A为2—Theta角为30°(d=2.97)时峰值的相对强度Ⅰ与半高宽的乘积,其计算误差仅6.32％。  相似文献   

一种改进的膜盐度计   总被引：1，自引：0，他引：1       下载免费PDF全文

陈国华  王立红  张力军  关庆利 《海洋科学》1993,17(5):54-58

叙述了一种可用于现场及实验室使用的新的浸入式电池膜盐度计的设计与结构。该膜盐度计传感器由下列电化学电池组成,用此传感器与微机或pH/离子计配合组成了膜盐度计。该仪器可用于近岸河口地区表层海水盐度监测并用于有潮河口底栖动物生态现场研究。仪器现场条件使用准确度约为盐度测量值1％。同时能用于Cl~-及其他离子的现场监测。  相似文献   

青海钩虾配合饲料对罗非鱼两品系生长效果的研究     

武云飞  韩凤进  江涛  孔晓瑜 《中国海洋大学学报(自然科学版)》2003,33(2):199-205

研究青海钩虾对罗非鱼两品系吉富罗非鱼和彩虹鲷生长的促进作用。实验用青海钩虾与面粉、麸皮和豆饼制成 5组 ( ～ )配合饲料及良种场原用饲料 ( 组 )饲育吉富罗非鱼和彩虹鲷 ,经2 5 d试验结果表明 :( 1)彩虹鲷增重效果以 组饲料最高 ,平均增重为 4 4 .9g,较大于 组的 4 3.1g;( 2 )所有钩虾饲料对彩虹鲷的增色效果都高于良种场的原用饲料 组 ,其中以饲料 组最高 ;( 3)饲料 I组饲育吉富罗非鱼平均增重为 4 4 g,高于 组的 4 0 .1g;( 4 )吉富罗非鱼和彩虹鲷比较则显示出吉富罗非鱼的增重率明显高于彩虹鲷而饵料系数又较低的结果。  相似文献